Tissue-based techniques in Toxicologic Pathology and Safety Pharmacology studies
arrow_back Tissue-based techniques in Toxicologic Pathology and Safety Pharmacology studies
Anfiteatro Farmacologia
Curso com Frédéric Gervais, DVM, DESVAP, DECVP (Citoxlab France), organizado pelo Programa de Pós-graduação em Farmacologia.
O CURSO É GRATUITO (VAGAS LIMITADAS).
Período: 21 a 23 de novembro de 2018, das 14h às 16:15h
Local: Departamento de Farmacologia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas
Público-alvo: Estudantes de graduação (últimos anos), estudantes de pós-graduação e docentes/pesquisadores interessados ou atuantes no tema
Atenção: Este curso será inteiramente em inglês. Não haverá tradução simultânea. A adesão ao curso será por palestra (adesão modular), sendo o certificado emitido conforme a adesão e a frequência.
Period: November 21-23 (2018), 14:00-16:15h
Place: Department of Pharmacology, Faculty of Medical Sciences (FCM), UNICAMP, Campinas, SP
Target audience: Advance undergraduate students, Post-graduate students and Researchers interested in the subject
Course Programme:
21/11, 14:00-16:15h
Importance of biomarkers in a Preclinical Contract Research Organization (CRO)
What’s a CRO? Presenting Citoxlab
Importance of biomarkers in drug development
Presentation of the different labs at Citoxlab (genomic, immuno, flow cytometry, clinical pathology, histopathology)
Coffee break
Formalin-fixed paraffin-embedded (FFPE) tissue blocks/sections: review of molecular pathology techniques (relevance and limitations)
Investigation of proteins, DNA and RNA (“in solution” and “in situ” techniques)
22/11, 14:00-16:15h
Immunohistochemistry: general principles and applications
Workflow: from sampling to reading
A special focus on parameters influencing reproducibility
Automation, frozen/FFPE, antigen retrieval, blocking etc.
Selection of antibodies/detection systems etc.
Coffee break
IHC method development/optimization and validation in the context of GLP-compliant Tissue Cross-reactivity (TCR) studies
What are biologics?
What are TCR studies?
How are TCR projects conducted?
Good Laboratory Pratice (GLP) context and consequences
23/11, 14:00-16:15h
Multiplexing and other tools in immunohistochemistry: principles
Strategy in multiplexing
Investigation of protein-protein interactions
Coffee break
In situ Hybridization: General principles and applications
General principles
Examples and applications of CISH/FISH techniques as diagnostic tools
microRNAs (miRs) as biomarkers
RNAscope: high sensitivity/specificity detection of mRNA in FFPE samples
Attention: This course will be entirely in English. There will be no simultaneous translation