Hydroelectrolytic Metabolism Laboratory Address Experimental Medicine and Surgery Nucleus School of Health Science College of Medicine at State University of Campinas, SP Contact Details Email: email@example.com and firstname.lastname@example.org Research Profile According to David Barker and colleagues (1989), changes in maternal environment can have great impact on organic development of the offspring. Both malnutrition and, more recently, obesity are known to affect the function of a number of organs and systems. Our research field of study focuses on different aspects of developmental biology and blood pressure control, especially on maternal environmental (nutritional) factors that impact on the kidney development and function and this would result in irreversible damage to adult offspring health. Since maternal obesity and diabetes is rapidly increasing in the world we evaluate the impact of nutrient excess and deficiency during gestation on the process of nephrogenesis in the offspring. This implies that normal variations in the transfer of food from mothers to babies have profound long-term implications for the health of the next generation. Our group has shown that low nephron number is linked with increased risk of blood pressure and abnormal kidney sodium handling in adulthood. Also, we have done studies demonstrating striking morphological and functional changes of autonomic control areas of central nervous system which are associated with fetal programming. We were the first to show the link between gestational low protein intake, podocytes disorders and abnormal nephron expression of angiotensin receptors, miRNA and enhanced collagen expression. Prior studies in our lab also have shown memory and learning changes associated with dendritic arborization in hippocampus e BNST cerebral structures in programmed offspring. For that, a model of gestational exposure to protein deficient and/or fat-rich diets is employed to study the impact of these environmental factors on kidney ontogeny, neuroendocrine regulation of renal/adrenal function and, on renal mediated thermogenesis. The morphological approach is done by light and confocal microscopy; Signal transduction is evaluated by immunoblot. Gene expression is studied by real-time PCR. ELISA technique is employed to determine hormone concentration. Brain-kidney connectivity is evaluated in models submitted to stereotaxic manipulation.
José Antonio Rocha Gontijo
Linhas de pesquisa:
Metabolismo Hidrossalino e Fisiopatologia da Hipertensão Arterial; Controle Neural do Rim; Programação Fetal;
Hipertensão arterial; controle neural do rim; natriurese; programação fetal.
Colaboração/convênio com entidades estrangeiras:
Universidade do Minho, Braga, Portugal (Doutoramento Sanduiche) Monash University; Sidney, Australia (Doutoramento Sanduiche); Southhampton University, Southhampton, Inglaterra (Doutoramento Sanduiche).
Principais equipamentos utilizados para o desenvolvimento das pesquisas:
The morphological approach is done by light and confocal microscopy; Signal transduction is evaluated by immunoblot. Gene expression is studied by real-time PCR. ELISA technique is employed to determine hormone concentration. Brain-kidney connectivity is evaluated in models submitted to stereotaxic manipulation and neural recorder.
Fundos de financiamento:
Ize Penhas de Lima
Patricia Aline Boer
FCM15 - NMCE - Núcleo de Medicina e Cirurgia Experimental
Rua Cinco de Junho
Cidade Universitária Zeferino Vaz