O Departamento de Farmacologia e o Programa de Pós-graduação em Farmacologia da Faculdade de Ciências Médicas (FCM) da Unicamp realizam, de 21 a 23 de novembro das 14h às 16h15, o curso em “Tissue-based techniques in Toxicologic Pathology and Safety Pharmacology studies”, com o patologista francês Frédéric Gervais, do grupo Citoxlab France.

Trata-se de curso gratuito, totalmente ministrado em inglês. As vagas são limitadas. A adesão será modular (por palestra), sendo o certificado emitido conforme a adesão e a frequência. Inscrição aqui.

Público-alvo: Alunos de graduação, pós-graduação, docentes e profissionais interessados ou atuantes na área.

Programação

21/11
14:00-16:15h

Importance of biomarkers in a Preclinical Contract Research Organization (CRO)

What’s a CRO? Presenting Citoxlab
Importance of biomarkers in drug development
Presentation of the different labs at Citoxlab (genomic, immuno, flow cytometry, clinical pathology, histopathology)

Coffee break

Formalin-fixed paraffin-embedded (FFPE) tissue blocks/sections: review of molecular pathology techniques (relevance and limitations)

Investigation of proteins, DNA and RNA (“in solution” and “in situ” techniques)

22/11
14:00-16:15h

Immunohistochemistry: general principles and applications

Workflow: from sampling to reading
A special focus on parameters influencing reproducibility
Automation, frozen/FFPE, antigen retrieval, blocking etc.
Selection of antibodies/detection systems etc.

Coffee break

IHC method development/optimization and validation in the context of GLP-compliant Tissue Cross-reactivity (TCR) studies

What are biologics?
What are TCR studies?
How are TCR projects conducted?
Good Laboratory Pratice (GLP) context and consequences

23/11
14:00-16:15h

Multiplexing and other tools in immunohistochemistry: principles  

Strategy in multiplexing
Investigation of protein-protein interactions

Coffee break

In situ Hybridization: General principles and applications

General principles
Examples and applications of CISH/FISH techniques as diagnostic tools
microRNAs (miRs) as biomarkers
RNAscope: high sensitivity/specificity detection of mRNA in FFPE samples